Article
Remodeling of sperm chromatin after fertilization involves nucleosomes formed by sperm histones H2A and H2B and two CS histone variants.
Journal of cellular biochemistry - 1 Jan 2002
Oliver M Isabel, Concha Carolina, Gutiérrez Soraya, Bustos Alejandra, Montecino Martín, Puchi Marcia, Imschenetzky María
Abstract excerpt
The composition of nucleosomes at an intermediate stage of male pronucleus formation was determined in sea urchins. Nucleosomes were isolated from zygotes harvested 10 min post-insemination, whole nucleoprotein particles were obtained from nucleus by nuclease digestion, and nucleosomes were subsequently purified by a sucrose gradient fractionation. The nucleosomes derived from male pronucleus were separated from...
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