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Article

Hybrid nucleoprotein particles containing a subset of male and female histone variants form during male pronucleus formation in sea urchins.

Journal of cellular biochemistry - 15 Dec 1996

Imschenetzky M, Oliver M I, Gutiérrez S, Morín V, Garrido C, Bustos A, Puchi M

Abstract excerpt

To determine the changes in chromatin organization during male pronucleus remodeling, we have compared the composition of nucleoprotein particles (NP-ps) resulting from digestion with endogenous nuclease (ENase) and with micrococcal nuclease (MNase). Whole nuclei were isolated from sea urchin gametes and zygotes containing partially decondensed (15 min postinsemination, p.i.) or a fully decondensed (40 min p.i.)...

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Identifiers

PMID: 8978455
DOI: 10.1002/(sici)1097-4644(19961215)63:4<385::aid-jcb1>3.0.co;2-p

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