Article
Hybrid nucleoprotein particles containing a subset of male and female histone variants form during male pronucleus formation in sea urchins.
Journal of cellular biochemistry - 15 Dec 1996
Imschenetzky M, Oliver M I, Gutiérrez S, Morín V, Garrido C, Bustos A, Puchi M
Abstract excerpt
To determine the changes in chromatin organization during male pronucleus remodeling, we have compared the composition of nucleoprotein particles (NP-ps) resulting from digestion with endogenous nuclease (ENase) and with micrococcal nuclease (MNase). Whole nuclei were isolated from sea urchin gam...
Topics
- Animals
- Blotting, Western
- Cell Nucleus
- Chromatin
- DNA
- Electrophoresis, Agar Gel
- Endonucleases
- Female
- Genetic Variation
- Histones
- Male
- Micrococcal Nuclease
- Nucleosomes
- Oocytes
- Sea Urchins
- Spermatozoa
- Zygote