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Production of Cloned Pigs from Cultured Fetal Fibroblast Cells

1 May 2002

Abstract excerpt

Somatic cell nuclear transfer was used to produce live piglets from cultured fetal fibroblast cells. This was achieved by exposing donor cell nuclei to oocyte cytoplasm for approximately 3 h before activation by chemical means. Initially, an experiment was performed to optimize a cell fusion system that prevented concurrent activation in the majority of recipient cytoplasts. Cultured fibroblast cells were fused...

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PMID: 11967188
DOI: 10.1095/biolreprod66.5.1283

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