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Article

Method of oocyte activation affects cloning efficiency in pigs

16 Dec 2008

Abstract excerpt

The following experiments compared the efficiency of three fusion/activation protocols following somatic cell nuclear transfer (SCNT) with porcine somatic cells transfected with enhanced green fluorescent protein driven by the chicken beta-actin/rabbit beta-globin hybrid promoter (pCAGG-EGFP). The three protocols included electrical fusion/activation (NT1), electrical fusion/activation followed by treatment with...

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PMID: 19090011
DOI: 10.1002/mrd.20987

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