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Transgenic pig expressing the enhanced green fluorescent protein produced by nuclear transfer using colchicine‐treated fibroblasts as donor cells

21 May 2002

Abstract excerpt

Fetal-derived fibroblast cells were transduced with replication defective vectors containing the enhanced green fluorescent protein (EGFP). The transgenic cells were treated with colchicine, which theoretically would synchronize the cells into G2/M stage, and then used as donor nuclei for nuclear transfer. The donor cells were transferred into the perivitalline space of enucleated in vitro matured porcine...

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PMID: 12112592
DOI: 10.1002/mrd.10146

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