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Article

Comparison of Electro-Fusion and Intracytoplasmic Nuclear Injection Methods in Pig Cloning

1 Dec 2003

Abstract excerpt

This paper methodologically compares the electro-fusion (EF) and intracytoplasmic injection (ICI) methods, as well as simultaneous fusion/activation (SA) and delayed activation (DA), in somatic nuclear transfer in pigs using fetal fibroblast cells. Comparison of the remodeling pattern of donor nuclei after nuclear transfer by ICI or EF showed that a high rate (80-100%) of premature chromosome condensation...

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PMID: 14733754
DOI: 10.1089/153623003772032862

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