Article
Cellular oxidant stress and advanced glycation endproducts of albumin: caveats of the dichlorofluorescein assay.
Archives of biochemistry and biophysics - 1 Apr 2002
Subramaniam Ram, Fan Xing-Jun, Scivittaro Vincenzo, Yang Jianqi, Ha Chung-Eun, Petersen Charles E, Surewicz Witold K, Bhagavan Nadhipuram V, Weiss Miriam F, Monnier Vincent M
Abstract excerpt
In order to understand the mechanism by which advanced glycation endproducts (AGEs) elicit oxidative stress, macrophage-like RAW264.7 cells were exposed to various AGE-albumins, and oxidant stress was estimated from the fluorescence of oxidized dichlorofluorescein using the microtiter plate assay. Strongest fluorescence was observed with methylglyoxal modified albumin (MGO-BSA) compared with native albumin....
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