The serotonin binding site of human and murine 5-HT2B receptors: molecular modeling and site-directed mutagenesis.

doi:10.1074/jbc.M200195200

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Article

The serotonin binding site of human and murine 5-HT2B receptors: molecular modeling and site-directed mutagenesis.

The Journal of biological chemistry - 10 May 2002

Manivet Philippe, Schneider Benoit, Smith Jeremy Christopher, Choi Doo-Sup, Maroteaux Luc, Kellermann Odile, Launay Jean-Marie

Abstract excerpt

Bacteriorhodopsin and rhodopsin crystal structures were used as templates to build structural models of the mouse and human serotonin (5-HT)-2B receptors (5-HT(2B)Rs). Serotonin was docked to the receptors, and the amino acids predicted to participate to its binding were subjected to mutagenesis. 5-HT binding affinity and 5-HT-induced inositol triphosphate production were measured in LMTK(-) cells transfected...

Topics

Amino Acid Sequence
Animals
Aspartic Acid
Binding Sites
Cell Membrane
Humans
Hydrogen-Ion Concentration
Kinetics
Ligands
Mice
Models, Molecular

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Molecular Sequence Data

Mutagenesis, Site-Directed

Mutation

Phenylalanine

Point Mutation

Protein Binding

Protein Structure, Tertiary

Source

Open in PubMed

Identifiers

PMID: 11859080
DOI: 10.1074/jbc.M200195200

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026.

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