Expression of the prodrug-activating enzyme DT-diaphorase via Ad5 delivery to human colon carcinoma cells in vitro.

doi:10.1038/sj.cgt.7700430

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Article

Expression of the prodrug-activating enzyme DT-diaphorase via Ad5 delivery to human colon carcinoma cells in vitro.

Cancer gene therapy - 1 Feb 2002

Misra Veet, Klamut Henry J, Rauth A M

Abstract excerpt

Intratumoral injection of recombinant adenoviral type 5 (Ad5) vectors that carry prodrug-activating enzymes like DT-diaphorase (DTD) could be used to selectively target tumor cells for chemotherapy. To demonstrate the feasibility of this approach, Ad5 vectors were constructed, which express human DTD minigenes for both wild-type and mutant (C-to-T change in nucleotide 609 in DTD cDNA) DTD under the control of the...

Topics

Adenoviridae
Antibiotics, Antineoplastic
Blotting, Western
Cell Division
Colonic Neoplasms
Gene Expression
Gene Transfer Techniques
Genetic Vectors
Humans
Mitomycin
Mutation
NAD(P)H Dehydrogenase (Quinone)
Prodrugs
Tumor Cells, Cultured
Tumor Stem Cell Assay
beta-Galactosidase

Source

Open in PubMed

Identifiers

PMID: 11857040
DOI: 10.1038/sj.cgt.7700430

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