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Article

LNA probes in a real-time TaqMan PCR assay for genotyping of Giardia duodenalis in wastewaters.

Journal of applied microbiology - 1 May 2010

Alonso J L, Amorós I, Cuesta G

Abstract excerpt

AIMS: This study describes an approach for genotyping Giardia cysts obtained from wastewater treatment plants (WTPs) in Spain using real-time PCR (qPCR) in combination with immunomagnetic beads. METHODS AND RESULTS: A 50-cycle amplification of a 74-bp fragment of the Giardia beta-giardin gene was adopted from a previous qPCR method. Additionally, two locked nucleic acid (LNA) probes were designed (LNA P434 P1 for...

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PMID: 19840182
DOI: 10.1111/j.1365-2672.2009.04559.x

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