Article
Engineered improvements in DNA-binding function of the MATa1 homeodomain reveal structural changes involved in combinatorial control.
Journal of molecular biology - 15 Feb 2002
Hart Beverly, Mathias Jonathan R, Ott David, McNaughton Lynn, Anderson Janet S, Vershon Andrew K, Baxter Susan M
Abstract excerpt
We have engineered enhanced DNA-binding function into the a1 homeodomain by making changes in a loop distant from the DNA-binding surface. Comparison of the free and bound a1 structures suggested a mechanism linking van der Waals stacking changes in this loop to the ordering of a final turn in the DNA-binding helix of a1. Inspection of the protein sequence revealed striking differences in amino acid identity at...
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