Article
Functional regions of rice heat shock protein, Oshsp16.9, required for conferring thermotolerance in Escherichia coli.
Plant physiology - 1 Feb 2002
Yeh Ching-Hui, Chen Yih-Ming, Lin Chu-Yung
Abstract excerpt
Rice (Oryza sativa) class I low-molecular mass (LMM) heat shock protein (HSP), Oshsp16.9, has been shown to be able to confer thermotolerance in Escherichia coli. To define the regions for this intriguing property, deletion mutants of this hsp have been constructed and overexpressed in E. coli XL1-blue cells after isopropyl beta-D-thioglactopyranoside induction. The deletion of amino acid residues 30 through 36...
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