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Article

Chemostat approach for the directed evolution of biodesulfurization gain-of-function mutants.

Applied and environmental microbiology - 1 Feb 2002

Arensdorf Joseph J, Loomis A Katrina, DiGrazia Philip M, Monticello Daniel J, Pienkos Philip T

Abstract excerpt

Chemostat enrichment is a classical microbiological method that is well suited for use in directed-evolution strategies. We used a two-phase sulfur-limited chemostat to select for gain-of-function mutants with mutations in the biodesulfurization (Dsz) system of Rhodococcus erythropolis IGTS8, enriching for growth in the presence of organosulfur compounds that could not support growth of the wild-type strain....

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Identifiers

PMID: 11823208
DOI: 10.1128/AEM.68.2.691-698.2002
PMCID: PMC126670

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

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