Article
Viability of escherichia coli cells under long-term cultivation in a rich nutrient medium.
Genes & genetic systems - 1 Oct 2001
Fukuda T, Nakahigashi K, Inokuchi H
Abstract excerpt
We investigated the viability of Escherichia coli cells during long-term cultivation in Brain Heart Infusion (BHI) medium and observed that the number of viable cells increased, then decreased, and increased again, in this medium, and finally the cells died out within about 10 days. This cell death may result from an increase in the pH of the medium. After repeated cultivation in BHI, bacterial cells that did not...
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