Article
Rational design of catechol-2, 3-dioxygenase for improving the enzyme characteristics.
Applied biochemistry and biotechnology - 1 Sept 2010
Wei Jiashi, Zhou Ying, Xu Tao, Lu Baorong
Abstract excerpt
Catechol-2, 3-dioxygenase (C23O) from Pseudomonas sp. CGMCC2953 identified in our laboratory, which is one of the key enzymes responsible for phenanthrene biodegradation, was expected to get better characteristics tolerant to environment for its further application. With the aim of improving the enzyme properties by introducing intermolecular disulfide bonds, X-ray structure of a C23O from Pseudomonas putida...
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