Article
Environment around the chromophore in pharaonis phoborhodopsin: mutation analysis of the retinal binding site.
Biochimica et biophysica acta - 1 Dec 2001
Shimono K, Ikeura Y, Sudo Y, Iwamoto M, Kamo N
Abstract excerpt
Phoborhodopsin (pR or sensory rhodopsin II, sRII) and pharaonis phoborhodopsin (ppR or pharaonis sRII, psRII) have a unique absorption maximum (lambda(max)) compared with three other archaeal rhodopsins: lambda(max) of pR and ppR is approx. 500 nm and of others (e.g. bacteriorhodopsin, bR) is 560-590 nm. To determine the residue contributing to the opsin shift from ppR to bR, we constructed various ppR mutants,...
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