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Article

Rapid diagnosis of leishmaniasis by fluorogenic polymerase chain reaction.

1 Nov 2001

Abstract excerpt

A fluorescent DNA probe (LEIS.P1) specific for a conserved region of the small-subunit ribosomal RNA gene of Leishmania and a pair of flanking primers (LEIS.U1 and LEIS.L1) were designed for use in a fluorogenic polymerase chain reaction. Optimal assay conditions with zero background were established to detect low levels of Leishmania from clinical samples. By use of this assay, we amplified DNA from 27 strains...

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Identifiers

PMID: 11716118
DOI: 10.4269/ajtmh.2001.65.583

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