Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Detection, differentiation, and quantitation of pathogenic leishmania organisms by a fluorescence resonance energy transfer-based real-time PCR assay.

Journal of clinical microbiology - 1 Apr 2003

Schulz Alexandra, Mellenthin Katja, Schönian Gabriele, Fleischer Bernhard, Drosten Christian

Abstract excerpt

Real-time technology eliminates many of the pitfalls of diagnostic PCR, but this method has not been applied to differentiation of Leishmania organisms so far. We have developed a real-time PCR that simultaneously detects, quantitates, and categorizes Leishmania organisms into three relevant groups causing distinct clinical pictures. The analytical sensitivity (detection rate of >or=95% at 94.1 parasites/ml of...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to DNA Probes
Share to DNA, Protozoan

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to DNA, Ribosomal

Share to Fluorescence Resonance Energy Transfer

Share to Leishmania

Share to Leishmaniasis

Share to RNA, Ribosomal, 18S

Share to Sensitivity and Specificity

Source

Open in PubMed Open full text

Identifiers

PMID: 12682141
DOI: 10.1128/JCM.41.4.1529-1535.2003
PMCID: PMC153923

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Detection, differentiation, and quantitation of pathogenic leishmania organisms by a fluorescence resonance energy transfer-based real-time PCR assay.PMID 12682141Read ↗

A FRET-Based Real-Time PCR Assay to Identify the Main Causal Agents of New World Tegumentary LeishmaniasisSemantic proximity Read ↗

Evaluation of FRET real-time PCR assay for rapid detection and differentiation of Plasmodium species in returning travellers and migrantsSemantic proximity Read ↗

Development of a real-time polymerase chain reaction assay for the quantification of Leishmania species and the monitoring of systemic distribution of the pathogenSemantic proximity Read ↗

SYBR Green-based Real-Time PCR targeting kinetoplast DNA can be used to discriminate between the main etiologic agents of Brazilian cutaneous and visceral leishmaniasesSemantic proximity Read ↗

An Optimized Real-Time qPCR Method for the Effective Detection of Human Malaria InfectionsSemantic proximity Read ↗

Real-Time Fluorescence Loop Mediated Isothermal Amplification for the Diagnosis of MalariaSemantic proximity Read ↗

Real-Time PCR for Detection and Identification of <i>Plasmodium</i> sppSemantic proximity Read ↗

High Resolution Melting Analysis Targeting hsp70 as a Fast and Efficient Method for the Discrimination of Leishmania SpeciesSemantic proximity Read ↗

Sensitivity of PCR and real-time PCR for the diagnosis of human visceral leishmaniasis using peripheral bloodSemantic proximity Read ↗

Quantification of Leishmania infantum DNA by a Real-Time PCR Assay with High SensitivitySemantic proximity Read ↗

Real-time PCR using FRET technology for Old World cutaneous leishmaniasis species differentiationSemantic proximity Read ↗

Real-Time Fluorescence-Based PCR for Detection of Malaria ParasitesSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.