Article
Single-Step Multiplex PCR Assay for Characterization of New World <i>Leishmania</i> Complexes
1 Jul 1998
Abstract excerpt
We have developed a PCR assay for one-step differentiation of the three complexes of New World Leishmania (Leishmania braziliensis, Leishmania mexicana, and Leishmania donovani). This multiplex assay is targeted to the spliced leader RNA (mini-exon) gene repeats of these organisms and can detect all three complexes simultaneously, generating differently sized products for each complex. The assay is specific to...
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