Article
Variation in 16S-23S rRNA intergenic spacer regions among Bacillus subtilis 168 isolates.
Molecular microbiology - 1 Oct 2001
Shaver Y J, Nagpal M L, Fox K F, Rudner R, Fox A
Abstract excerpt
The genome of the Bacillus subtilis 168-type strain contains 10 ribosomal RNA (rRNA) operons. In the intergenic spacer region (ISR) between the 16S and 23S rRNA genes, five rRNA operons, rrnI-H-G and rrnJ-W, lack a trinucleotide signature region. Precise determination of molecular weight (MW), using electrospray mass spectrometry (MS), of the polymerase chain reaction (PCR) products from a segment of the ISR from...
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