Article
Effect of harvesting and sorting on beta-1 integrin in canine microvascular cells.
The Journal of surgical research - 1 Oct 2001
Piercy K T, Donnell R L, Kirkpatrick S S, Mundy B L, Stevens S L, Freeman M B, Goldman M H
Abstract excerpt
BACKGROUND: The goal of seeding prosthetic conduits with endothelial cells (ECs) has focused attention on the role of EC adhesion molecules. Cell preparation techniques may affect adhesion molecule expression and graft seeding. METHODS: Using fluorescent antibody labeling and flow cytometric analysis, this study examined the effectsof monolayer detachment (scraping vs trypsinization), timing of immunolabeling...
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