Article
Restoration of nucleotide excision repair in a helicase-deficient XPD mutant from intragenic suppression by a trichothiodystrophy mutation.
Molecular and cellular biology - 1 Nov 2001
George J W, Salazar E P, Vreeswijk M P, Lamerdin J E, Reardon J T, Zdzienicka M Z, Sancar A, Kadkhodayan S, Tebbs R S, Mullenders L H, Thompson L H
Abstract excerpt
The UV-sensitive V-H1 cell line has a T46I substitution mutation in the Walker A box in both alleles of XPD and lacks DNA helicase activity. We characterized three partial revertants that curiously display intermediate UV cytotoxicity (2- to 2.5-fold) but normal levels of UV-induced hprt mutations. In revertant RH1-26, the efficient removal of pyrimidine (6-4) pyrimidone photoproducts from both strands of hprt...
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