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DNA microarray analysis of Bacillus subtilis DegU, ComA and PhoP regulons: an approach to comprehensive analysis of B.subtilis two-component regulatory systems.

Nucleic acids research - 15 Sept 2001

Ogura M, Yamaguchi H, Yoshida Ki, Fujita Y, Tanaka T

Abstract excerpt

We have analyzed the regulons of the Bacillus subtilis two-component regulators DegU, ComA and PhoP by using whole genome DNA microarrays. For these experiments we took the strategy that the response regulator genes were cloned downstream of an isopropyl-beta-D-thiogalactopyranoside-inducible promoter on a multicopy plasmid and expressed in disruptants of the cognate sensor kinase genes, degS, comP and phoR,...

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PMID: 11557812
DOI: 10.1093/nar/29.18.3804
PMCID: PMC55910

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