Article
Thiamin auxotrophy in yeast through altered cofactor dependence of the enzyme acetohydroxyacid synthase.
Microbiology (Reading, England) - 1 Sept 2001
Byrne Kerry L, Meacock Peter A
Abstract excerpt
The THI1 gene of Saccharomyces cerevisiae has been identified and found to be allelic with the previously characterized gene ILV2 that encodes acetohydroxyacid synthase (AHAS). This enzyme catalyses the first step in the parallel biosyntheses of the branched-chain amino acids isoleucine and valin...
Topics
- Acetolactate Synthase
- Alleles
- Amino Acid Sequence
- Base Sequence
- Catalytic Domain
- Cloning, Molecular
- DNA, Fungal
- DNA-Binding Proteins
- Genes, Fungal
- Models, Molecular
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Phenotype
- Plasmids
- Saccharomyces cerevisiae
- Sequence Homology, Amino Acid
- Thiamine
- Thiamine Pyrophosphate