Article
Heterologous expression in budding yeast as a tool for studying the plant cell morphogenesis machinery.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2014
Cvrčková Fatima, Hála Michal
Abstract excerpt
The budding yeast (Saccharomyces cerevisiae) can serve as a unique experimental system for functional studies of heterologous genes, allowing not only complementation of readily available yeast mutations but also generation of overexpression phenotypes and in some cases also rescue of such phenotypes. Here we summarize the main considerations that have to be taken into account when using the yeast expression...
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