Article
An ancient player unmasked: T4 rI encodes a t-specific antiholin.
Molecular microbiology - 1 Aug 2001
Ramanculov E, Young R
Abstract excerpt
Phage T4 effects lysis by its holin T and its endolysin E. Lysis is inhibited (LIN) if the infected cell is subjected to secondary infections by T4 phage particles. The T4 rI gene is required for LIN in all hosts tested. Here, we show that a cloned rI gene can impose a T-specific LIN on T-mediated lysis in the context of the phage lambda infective cycle, in the absence of other T4 genes and without secondary...
Topics
- Alleles
- Amino Acid Sequence
- Bacteriophage T4
- Bacteriophage lambda
- Blotting, Western
- Cloning, Molecular
- Cross-Linking Reagents
- Escherichia coli
- Formaldehyde
- Gene Expression Regulation, Viral
- Genes, Viral
- Lysogeny
- Molecular Sequence Data
- Nickel
- Signal Transduction
- Substrate Specificity
- Viral Proteins