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Identification of 54 Mycobacterial Species by PCR-Restriction Fragment Length Polymorphism Analysis of the <i>hsp65</i> Gene

1 Aug 2001

Abstract excerpt

A total of 121 reference and clinical strains of both slowly and rapidly growing mycobacteria belonging to 54 species were studied for restriction fragment length polymorphism of a PCR-amplified 439-bp segment of the gene encoding the 65-kDa heat shock protein. Restriction digests were separated by 10% polyacrylamide gel electrophoresis (PAGE). By including a size standard in each sample, the restriction fragment...

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PMID: 11473995
DOI: 10.1128/jcm.39.8.2799-2806.2001

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