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Species Identification of Mycobacteria by PCR-Restriction Fragment Length Polymorphism of the <i>rpoB</i> Gene

1 Aug 2000

Abstract excerpt

PCR-restriction fragment length polymorphism analysis (PRA) using the novel region of the rpoB gene was developed for rapid and precise identification of mycobacteria to the species level. A total of 50 mycobacterial reference strains and 3 related bacterial strains were used to amplify the 360-bp region of rpoB, and the amplified DNAs were subsequently digested with restriction enzymes such as MspI and HaeIII....

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PMID: 10921960
DOI: 10.1128/jcm.38.8.2966-2971.2000

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