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Use of PCR-Restriction Enzyme Pattern Analysis and Sequencing Database for <i>hsp65</i> Gene-Based Identification of <i>Nocardia</i> Species

1 Feb 2006

Abstract excerpt

Nocardia identification required laborious and time-consuming phenotypic and chemotaxonomic methods until molecular methods were developed in the mid-1990s. Here we reassessed the capacity of PCR-restriction enzyme pattern analysis (PRA) of the hsp65 gene to differentiate Nocardia species, including 36 new species. Our results confirm that hsp65 PRA must no longer be used for Nocardia species identification, as...

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PMID: 16455910
DOI: 10.1128/jcm.44.2.536-546.2006

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