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Rapid identification of mycobacteria to the species level by polymerase chain reaction and restriction enzyme analysis

1 Feb 1993

Abstract excerpt

A method for the rapid identification of mycobacteria to the species level was developed on the basis of evaluation by the polymerase chain reaction (PCR) of the gene encoding for the 65-kDa protein. The method involves restriction enzyme analysis of PCR products obtained with primers common to all mycobacteria. Using two restriction enzymes, BstEII and HaeIII, medically relevant and other frequent laboratory...

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PMID: 8381805
DOI: 10.1128/jcm.31.2.175-178.1993

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