Article
Isolation of precise plastid deletion mutants by homology-based excision: a resource for site-directed mutagenesis, multi-gene changes and high-throughput plastid transformation.
The Plant journal : for cell and molecular biology - 1 Jun 2006
Kode Vasumathi, Mudd Elisabeth A, Iamtham Siriluck, Day Anil
Abstract excerpt
We describe a simple and efficient homology-based excision method to delete plastid genes. The procedure allows one or more adjacent plastid genes to be deleted without the retention of a marker gene. We used aadA-based transformation to duplicate a 649 bp region of plastid DNA corresponding to the atpB promoter region. Efficient recombination between atpB repeats deletes the intervening foreign genes and 1,984...
Topics
- Alleles
- Base Sequence
- Gene Deletion
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Phenotype
- Photosynthesis
- Plants, Genetically Modified
- Plastids
- Ribulose-Bisphosphate Carboxylase
- Sequence Alignment
- Sequence Analysis, DNA
- Nicotiana