Article
Incorporating the ABI GeneScan analysis to a RACE-based technique for mapping multiple transcription initiation sites.
Molecular biotechnology - 1 Feb 2001
Yam J W, Chan K W, Hsiao W L
Abstract excerpt
Determination of transcription initiation sites has commonly been performed by primer extension and RNase protection assay using radioactively labeled oligonucleotides. Recently, a protocol based on modified 5' rapid amplification of cDNA ends (RACE) with the use of fluorescently labeled primer was developed. Here, we describe the use of RACE-based technique in conjunction with the GeneScan analysis for the...
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