Article
Accurate prediction of macrolide resistance in Helicobacter pylori by a PCR line probe assay for detection of mutations in the 23S rRNA gene: multicenter validation study.
Antimicrobial agents and chemotherapy - 1 May 2001
van Doorn L J, Glupczynski Y, Kusters J G, Mégraud F, Midolo P, Maggi-Solcà N, Queiroz D M, Nouhan N, Stet E, Quint W G
Abstract excerpt
Helicobacter pylori strains from 299 patients were tested in six laboratories in different countries. Macrolide susceptibility of the strains was determined by agar dilution (17.4%) or the epsilometer test (82.6%). Mutations in the 23S ribosomal DNA (rDNA) that are associated with macrolide resistance were analyzed by PCR and reverse hybridization (PCR-line probe assay [LiPA]). This method identifies A2115G,...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.