Article
Mapping of the Rsd contact site on the sigma 70 subunit of Escherichia coli RNA polymerase.
Journal of bacteriology - 1 May 2001
Jishage M, Dasgupta D, Ishihama A
Abstract excerpt
Rsd (regulator of sigma D) is an anti-sigma factor for the Escherichia coli RNA polymerase sigma(70) subunit. The contact site of Rsd on sigma(70) was analyzed after mapping of the contact-dependent cleavage sites by Rsd-tethered iron-p-bromoacetamidobenzyl EDTA and by analysis of the complex formation between Ala-substituted sigma(70) and Rsd. Results indicate that the Rsd contact site is located downstream of...
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