Article
Selection of ligands by panning of domain libraries displayed on phage lambda reveals new potential partners of synaptojanin 1.
Journal of molecular biology - 13 Apr 2001
Zucconi A, Dente L, Santonico E, Castagnoli L, Cesareni G
Abstract excerpt
One of the goals of functional genomics is the description of reliable and complete protein interaction networks. To facilitate ligand discovery from complex protein mixtures, we have developed an improved approach that is affected by a negligible fraction of false positives. We have combined a novel technique based on the display of cDNA libraries on the capsid of bacteriophage lambda and an efficient plaque...
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