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Article

Mapping protein interactions by combining antibody affinity maturation and mass spectrometry

5 Jun 2011

Abstract excerpt

Mapping protein interactions by immunoprecipitation is limited by the availability of antibodies recognizing available native epitopes within protein complexes with sufficient affinity. Here we demonstrate a scalable approach for generation of such antibodies using phage display and affinity maturation. We combined antibody variable heavy (V(H)) genes from target-specific clones (recognizing Src homology 2 (SH2)...

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Share to Advanced Biosensing Techniques and Applications
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Identifiers

PMID: 21704603
DOI: 10.1016/j.ab.2011.05.005

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