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Article

Substrate-induced activation of a trapped IMC-mediated protein folding intermediate.

Nature structural biology - 1 Apr 2001

Inouye M, Fu X, Shinde U

Abstract excerpt

While several unfolded proteins acquire native structures through distinct folding intermediates, the physiological relevance and importance of such states in the folding kinetics remain controversial. The intramolecular chaperone (IMC) of subtilisin was used to trap a partially folded, stable crosslinked intermediate conformer (CLIC) through a disulfide bond between mutated IMC and subtilisin. The trapped CLIC...

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PMID: 11276251
DOI: 10.1038/86194

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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