Article
Snapshots of DsbA in action: detection of proteins in the process of oxidative folding.
Science (New York, N.Y.) - 23 Jan 2004
Kadokura Hiroshi, Tian Hongping, Zander Thomas, Bardwell James C A, Beckwith Jon
Abstract excerpt
DsbA, a thioredoxin superfamily member, introduces disulfide bonds into newly translocated proteins. This process is thought to occur via formation of mixed disulfide complexes between DsbA and its substrates. However, these complexes are difficult to detect, probably because of their short-lived nature. Here we show that it is possible to detect such covalent intermediates in vivo by a mutation in DsbA that...
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