Article
Allele-specific detection of nascent transcripts by fluorescence in situ hybridization reveals temporal and culture-induced changes in Igf2 imprinting during pre-implantation mouse development.
Genes to cells : devoted to molecular & cellular mechanisms - 1 Mar 2001
Ohno M, Aoki N, Sasaki H
Abstract excerpt
BACKGROUND: Genomic imprinting causes parental-origin-specific monoallelic transcription of a subset of mammalian genes in the embryo and adult. There is conflicting evidence, however, for the monoallelic transcription of some imprinted genes, such as Igf2, in pre-implantation embryos. RESULTS: We have developed an allele-specific fluorescence in situ hybridization method which involves a pair of oligonucleotide...
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