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Article

Assessment of bacterial community structure in soil by polymerase chain reaction and denaturing gradient gel electrophoresis.

Journal of microbiological methods - 1 Oct 1999

Gelsomino A, Keijzer-Wolters A C, Cacco G, van Elsas J D

Abstract excerpt

Bacterial community structure was studied in a Flevo silt loam (FSL) soil microplot, as well as in 15 other soils, by using DNA extraction followed by molecular fingerprinting. Total community DNA was extracted and purified by a direct method, which yielded amplifiable DNA of high molecular weight for all soils. A variable region of the 16S rRNA gene was then amplified by PCR with bacterial primers, resulting in...

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PMID: 10520580
DOI: 10.1016/s0167-7012(99)00054-8

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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