Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

[Expression and purification of recombinant hirudin].

Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi - 1 Mar 1998

Guo J, Wang H, Gu J

Abstract excerpt

OBJECTIVE: To express the recombinant hirudin variant 1 (rHV1) with biological activity in prokaryotic cells and then isolate and purify the expressed products. METHODS: The hirudin variant 1 gene in plasmid vector pBV220 was expressed in E. coli strain DH5 alpha. The biological activity of rHV1...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Escherichia coli
Share to Fibrinolytic Agents
Share to Gene Expression

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to SDS gene

Share to Genetic Variation

Share to Recombinant Proteins

Source

Identifiers

PMID: 11243147

Genes

SDS

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 1 Sept 2026. Prepared record publication-11243147-2026-09-01.1. Manifest publication-graph-cohort-2026-09-01.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 1 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

[Expression and purification of recombinant hirudin].PMID 11243147Read ↗

Cloning, Characterization, and Heterologous Expression of a Candidate Hirudin Gene from the Salivary Gland Transcriptome of Hirudo nipponiaSemantic proximity Read ↗

Hirudin variants production by genetic engineered microbial factory.Semantic proximity Read ↗

Diversity of hirudin and hirudin-like factor genes in the North-African medicinal leech, Hirudo troctina.Semantic proximity Read ↗

Expression, purification and characterization of multigram amounts of a recombinant hybrid HV1-HV2 hirudin variant expressed in Saccharomyces cerevisiae.Semantic proximity Read ↗

Expression screening and functional verification of recombinant hirudin from novel chassis cell <i>Chlamydomonas reinhardtii</i>Semantic proximity Read ↗

Potent antithrombin activity and delayed clearance from the circulation characterize recombinant hirudin genetically fused to albumin.Semantic proximity Read ↗

Directed evolution towards protease-resistant hirudin variants.Semantic proximity Read ↗

The development of hirudin as an antithrombotic drug.Semantic proximity Read ↗

Inhibition of thrombin by hirudin genetically fused to wild-type or mutant antithrombin.Semantic proximity Read ↗

[Construction and application of a new prokaryotic expression vector derivative of pBV220].Semantic proximity Read ↗

Anti-hirudin monoclonal antibodies directed toward discontinuous epitopes of the hirudin amino-terminal and epitopes involving the carboxy-terminal hirudin amino acids.Semantic proximity Read ↗

Characterization of the deamidated forms of recombinant hirudin.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.