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[Expression and purification of recombinant hirudin].

Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi - 1 Mar 1998

Guo J, Wang H, Gu J

Abstract excerpt

OBJECTIVE: To express the recombinant hirudin variant 1 (rHV1) with biological activity in prokaryotic cells and then isolate and purify the expressed products. METHODS: The hirudin variant 1 gene in plasmid vector pBV220 was expressed in E. coli strain DH5 alpha. The biological activity of rHV1 was determined with chromogenic substrate method. The expressed product was purified by ultrafiltration, DEAE-Sephadex...

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PMID: 11243147

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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