Article
Plant microsatellite genotyping with 4-color fluorescent detection using multiple-tailed primers.
Genetics and molecular research : GMR - 31 Mar 2006
Missiaggia Alexandre, Grattapaglia Dario
Abstract excerpt
We extended the concept of fluorescent microsatellite genotyping with a single-universal tailed primer to the simultaneous use of three different tailed primers to allow multiplexed 4-color detection for medium throughput genotyping of plant species. The method was tested on Eucalyptus DNA samples using three forward primer sequences of human microsatellite markers labeled with different fluorescent dyes. The...
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