Article
Construction of phosphatidylethanolamine-less strain of Saccharomyces cerevisiae. Effect on amino acid transport.
Yeast (Chichester, England) - 1 Feb 2001
Robl I, Grassl R, Tanner W, Opekarová M
Abstract excerpt
A triple yeast mutant was constructed which lacks BST1, the gene for sphingosine lyase, besides the phosphatidylserine decarboxylases PSD1 and PSD2. In this yeast mutant, which can only be grown in the presence of exogenous ethanolamine, phosphatidylethanolamine can be depleted to very low levels. Under those conditions, respiration as well as glucose and 3-O-methylglucose uptake proceed unaffected. Plasma...
Topics
- Adenosine Triphosphatases
- Amino Acid Transport Systems
- Amino Acids
- Biological Transport
- Blotting, Western
- Carboxy-Lyases
- Carrier Proteins
- Cell Membrane
- Chromatography, Thin Layer
- DNA Primers
- DNA, Fungal