Article
LD-PCR coupled to long-read direct sequencing: an approach for mutation detection in genes with compact genomic structures.
Journal of biochemical and biophysical methods - 30 Jan 2001
Fleming N, Maynard J, Tzitzis L, Sampson J R, Cheadle J P
Abstract excerpt
A number of techniques have been developed as primary screens to scan for DNA sequence variants, including denaturing gradient gel electrophoresis, denaturing high-performance liquid chromatography, single-strand conformation polymorphism and heteroduplex analysis. Variant alleles detected by these assays are subsequently characterised by DNA sequencing. Sequencing itself is rarely used as a primary screen...
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