Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Bifidobacterial diversity in human feces detected by genus-specific PCR and denaturing gradient gel electrophoresis.

Applied and environmental microbiology - 1 Feb 2001

Satokari R M, Vaughan E E, Akkermans A D, Saarela M, de Vos W M

Abstract excerpt

We describe the development and validation of a method for the qualitative analysis of complex bifidobacterial communities based on PCR and denaturing gradient gel electrophoresis (DGGE). Bifidobacterium genus-specific primers were used to amplify an approximately 520-bp fragment from the 16S ribosomal DNA (rDNA), and the fragments were separated in a sequence-specific manner in DGGE. PCR products of the same...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Bifidobacterium
Share to DNA, Ribosomal

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Electrophoresis, Polyacrylamide Gel

Share to Genes, rRNA

Share to RNA, Ribosomal, 16S

Share to Sequence Analysis, DNA

Share to Species Specificity

Source

Open in PubMed Open full text

Identifiers

PMID: 11157210
DOI: 10.1128/AEM.67.2.504-513.2001
PMCID: PMC92614

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Bifidobacterial diversity in human feces detected by genus-specific PCR and denaturing gradient gel electrophoresis.PMID 11157210Read ↗

Evaluation of genetic diversity among strains of the human gut commensal Bifidobacterium adolescentis.Semantic proximity Read ↗

Assessment of genome evolution in Bifidobacterium adolescentis indicates genetic adaptation to the human gut.Semantic proximity Read ↗

Multiplex PCR using 16S rRNA gene-targeted primers for the identification of bifidobacteria from human originSemantic proximity Read ↗

Comparison of DNA extraction kits for PCR-DGGE analysis of human intestinal microbial communities from fecal specimensSemantic proximity Read ↗

Terminal Restriction Fragment Length Polymorphism Analysis for Human Fecal Microbiota and Its Application for Analysis of Complex Bifidobacterial CommunitiesSemantic proximity Read ↗

Exploring the diversity of the bifidobacterial population in the human intestinal tract.Semantic proximity Read ↗

Exploration of the genomic diversity and core genome of the Bifidobacterium adolescentis phylogenetic group by means of a polyphasic approach.Semantic proximity Read ↗

Intestinal Protozoan Infections Shape Fecal Bacterial Microbiota in Children from Guinea-Bissau.Semantic proximity Read ↗

Development of a Novel Long-Range 16S rRNA Universal Primer Set for Metagenomic Analysis of Gastrointestinal Microbiota in Newborn InfantsSemantic proximity Read ↗

Temporal stability analysis of the microbiota in human feces by denaturing gradient gel electrophoresis using universal and group-specific 16S rRNA gene primersSemantic proximity Read ↗

Development of 16S rRNA-Gene-Targeted Group-Specific Primers for the Detection and Identification of Predominant Bacteria in Human FecesSemantic proximity Read ↗

Evaluation of bifidobacterial community composition in the human gut by means of a targeted amplicon sequencing (ITS) protocolSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.