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Multiplex PCR using 16S rRNA gene-targeted primers for the identification of bifidobacteria from human origin

1 May 2003

Abstract excerpt

Three multiplex polymerase chain reactions (PCRs) targeted on Bifidobacterium and related species were designed to identify human species. The selected primers yielded amplified products of various sizes, each specific for a species. Three to four pairs were gathered in one PCR reaction and their specificity under multiplex conditions was confirmed using DNA from 26 reference strains. Using this technique on...

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PMID: 12757956
DOI: 10.1016/s0378-1097(03)00245-3

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