Sensitive and Specific Method for Rapid Identification of <i>Streptococcus pneumoniae</i> Using Real-Time Fluorescence PCR

Abstract excerpt

Molecular surveillance of pathogens has shown the need for rapid and dependable methods for the identification of organisms of clinical and epidemiological importance. As the leading cause of community-acquired pneumonia, Streptococcus pneumoniae was used as a model organism to develop and refine a real-time fluorescence PCR assay and enhanced DNA purification method. Seventy clinical isolates of S. pneumoniae,...

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