Article
Assessment of clone identity and sequence fidelity for 1189 IMAGE cDNA clones.
Nucleic acids research - 15 Jan 2001
Halgren R G, Fielden M R, Fong C J, Zacharewski T R
Abstract excerpt
This report documents the error rate in a commercially distributed subset of the IMAGE Consortium mouse cDNA clone collection. After isolation of plasmid DNA from 1189 bacterial stock cultures, only 62. 2% were uncontaminated and contained cDNA inserts that had significant sequence identity to published data for the ordered clones. An agarose gel electrophoresis pre-screening strategy identified 361 stock...
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