Article
Cloning and functional studies of a luxO regulator LuxT from Vibrio harveyi.
Biochimica et biophysica acta - 1 Dec 2000
Lin Y H, Miyamoto C, Meighen E A
Abstract excerpt
LuxO is the central regulator integrating the quorum sensing signals controlling autoinduction of luminescence in Vibrio harveyi. We have previously purified to homogeneity a new lux regulator, LuxT, that binds to the luxO promoter. Based on the sequence of the tryptic peptides of LuxT, degenerate oligonucleotides were designed for PCR of the genomic DNA. A 273 bp PCR DNA fragment containing sequences encoding...
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