Article
Yeast nuclear pore complex assembly defects determined by nuclear envelope reconstruction.
Journal of structural biology - 1 Oct 2000
Gomez-Ospina N, Morgan G, Giddings T H, Kosova B, Hurt E, Winey M
Abstract excerpt
Assembly of nuclear pore complexes (NPCs) is a critical yet poorly understood cellular function. One approach to studying NPC assembly is to identify yeast mutants defective in this process. This requires robust assays for NPC assembly that can be used for phenotypic analysis. We have previously reconstructed yeast nuclei from electron micrographs of serially sectioned cells to precisely determine the number of...
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